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. 2021 Jun 25;9(6):e002373. doi: 10.1136/jitc-2021-002373

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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PM21-NK cells combine with P/V virus infection to increase killing of three-dimensional (3D) spheroid cultures of A549 lung cancer cells. Spheroid 3D cultures of A549-NLR cells were mock infected or infected with P/V virus at a multiplicity of infection of 50. At 16 hpi, PM21-NK cells were added to the cultures at E:T ratios of 1.25, 2.5 or 5 and red image fluorescence (total red integrated intensity (TRII)) was calculated from images recorded on the IncuCyte instrument at 4-hour intervals. (A) Representative spheroids pictures recorded at 4, 24 and 44 hours postaddition of NK cells at E:T ratios of 1.25, 2.5 and 5. (B and C) Time-dependent changes in red image fluorescence (TRII) expressed as per cent of time 0 when PM21-NK cells were added to culture are shown for E:T ratios of 2.5 (panel B) and 1.25 (panel C). Data are from six replicate cultures. E:T, effector:target; NK, natural killer.