Table 4. Amino acid concentration (g 100 g⁻¹ of dry weight) of mitten crab muscle from each site and sex (mean ± SE).

	Suqian		Guannan		Sihong		Xinghua		Gaochun		Jintan		Suzhou		Effect test, p
	♂	♀	♂	♀	♂	♀	♂	♀	♂	♀	♂	♀	♂	♀	Location	Sex	Location × Sex
Asp	6.99 ± 0.17ab	7.3 ± 0.2ab	7.27 ± 0.02a	7.3 ± 0.1a	6.69 ± 0.08b	7.04 ± 0.04b	7.1 ± 0.2ab	7.23 ± 0.05ab	7.44 ± 0.04a	7.2 ± 0.2a	7.32 ± 0.03a	7.24 ± 0.02a	7.16 ± 0.03ab	7.05 ± 0.02ab	0.017	0.366	0.142
Thr	3.47 ± 0.07a	3.56 ± 0.04a	3.52 ± 0.03a	3.51 ± 0.04a	3.24 ± 0.04b	3.43 ± 0.04b	3.46 ± 0.05a	3.56 ± 0.01a	3.74 ± 0.03a	3.51 ± 0.03a	3.59 ± 0.02a	3.6 ± 0.03a	3.52 ± 0.02a	3.54 ± 0.04a	0.000	0.248	0.003
Ser	2.9 ± 0.3	2.92 ± 0.02	2.91 ± 0.03	2.91 ± 0.03	2.73 ± 0.03	2.85 ± 0.04	2.89 ± 0.01	2.95 ± 0.03	3.18 ± 0.03	2.85 ± 0.02	3.08 ± 0.02	2.98 ± 0.02	2.96 ± 0.05	2.95 ± 0.02	0.176	0.462	0.25
Glu	10.85 ± 0.29b	10.84 ± 0.02b	10.75 ± 0.35b	10.85 ± 0.3b	10.45 ± 0.03b	10.68 ± 0.02b	10.95 ± 0.04b	10.83 ± 0.06b	12.11 ± 0.03a	10.92 ± 0.02a	11.65 ± 0.05a	11.27 ± 0.03a	12.06 ± 0.03a	11.42 ± 0.02a	0.000	0.003	0.004
Gly	4.69 ± 0.26c	4.67 ± 0.06c	5.01 ± 0.19a	4.99 ± 0.32a	5.19 ± 0.04a	5.13 ± 0.03a	5.12 ± 0.02b	4.6 ± 0.1b	4.56 ± 0.03c	4.05 ± 0.02c	5.33 ± 0.03b	4.76 ± 0.02b	5.24 ± 0.04a	5.03 ± 0.03a	0.000	0.001	0.141
Ala	6.52 ± 0.02c	6.45 ± 0.03c	7.01 ± 0.09bc	6.48 ± 0.14bc	7.2 ± 0.2a	7.32 ± 0.04a	6.47 ± 0.03b	7.37 ± 0.07b	6.61 ± 0.04cd	6.12 ± 0.02cd	6.54 ± 0.02cd	6.3 ± 0.05cd	6.28 ± 0.06d	6.13 ± 0.03d	0.000	0.139	0.000
Cys	1.27 ± 0.06ab	1.29 ± 0.07ab	1.27 ± 0.07ab	1.35 ± 0.05ab	1.27 ± 0.05a	1.41 ± 0.01a	1.34 ± 0.02a	1.31 ± 0.06a	1.13 ± 0.04ab	1.36 ± 0.03ab	1.01 ± 0.01ab	1.38 ± 0.01ab	1.22 ± 0.04b	1.13 ± 0.03b	0.012	0.001	0.002
Val	2.91 ± 0.03b	3.22 ± 0.02b	3.12 ± 0.04b	3 ± 0.15b	3.02 ± 0.02b	2.98 ± 0.04b	3.11 ± 0.04b	2.99 ± 0.1b	3.42 ± 0.03a	3.28 ± 0.01a	3.3 ± 0.1ab	3.26 ± 0.01ab	3.27 ± 0.07ab	3.24 ± 0.04ab	0.000	0.465	0.046
Met	2.05 ± 0.05a	2.09 ± 0.09a	1.99 ± 0.07ab	1.88 ± 0.09ab	1.83 ± 0.03b	1.89 ± 0.06b	2.1 ± 0.1a	2.03 ± 0.03a	2.03 ± 0.04ab	1.84 ± 0.01ab	1.91 ± 0.03ab	1.88 ± 0.04ab	1.86 ± 0.03ab	1.9 ± 0.05ab	0.01	0.248	0.327
Ile	3.15 ± 0.04bc	3.26 ± 0.05bc	3.25 ± 0.05bc	3.19 ± 0.06bc	3.03 ± 0.01c	3.16 ± 0.02c	3.06 ± 0.02c	3.16 ± 0.03c	3.52 ± 0.04a	3.34 ± 0.02a	3.41 ± 0.03a	3.38 ± 0.03a	3.28 ± 0.06b	3.24 ± 0.02b	0.000	0.829	0.007
Leu	5.31 ± 0.03c	5.51 ± 0.01c	5.43 ± 0.06c	5.46 ± 0.02c	5.11 ± 0.01d	5.24 ± 0.04d	5.33 ± 0.03c	5.45 ± 0.04c	6.06 ± 0.02a	5.68 ± 0.04a	5.81 ± 0.03a	5.74 ± 0.04a	5.61 ± 0.01b	5.63 ± 0.03b	0.000	0.676	0.000
Tyr	2.93 ± 0.04ab	3.02 ± 0.02ab	2.92 ± 0.03ab	3.09 ± 0.01ab	2.73 ± 0.02c	2.77 ± 0.04c	2.88 ± 0.07b	2.98 ± 0.08b	3.12 ± 0.01a	3.06 ± 0.01a	3.12 ± 0.04a	2.93 ± 0.03a	2.9 ± 0.02b	2.88 ± 0.08b	0.000	0.388	0.008
Phe	2.94 ± 0.08b	3.03 ± 0.03b	3.16 ± 0.03a	3.32 ± 0.03a	2.93 ± 0.02b	3.03 ± 0.02b	3.09 ± 0.08b	2.98 ± 0.04b	3.39 ± 0.02a	3.18 ± 0.02a	3.17 ± 0.02a	3.23 ± 0.03a	3.24 ± 0.02a	3.21 ± 0.07a	0.000	0.708	0.007
Lys	5.62 ± 0.07a	5.77 ± 0.05a	5.9 ± 0.4a	5.77 ± 0.06a	5.39 ± 0.04a	5.64 ± 0.03a	5.73 ± 0.03a	5.79 ± 0.04a	5.96 ± 0.02a	5.08 ± 0.04a	5.52 ± 0.05a	5.39 ± 0.02a	5.5 ± 0.05b	5.29 ± 0.09b	0.015	0.062	0.006
His	1.68 ± 0.01a	1.6 ± 0.05a	1.66 ± 0.1a	1.67 ± 0.05a	1.5 ± 0.1a	1.58 ± 0.03a	1.66 ± 0.04a	1.59 ± 0.04a	1.47 ± 0.01a	1.62 ± 0.02a	1.54 ± 0.04a	1.58 ± 0.01a	1.45 ± 0.05b	1.46 ± 0.06b	0.026	0.509	0.423
Arg	6.95 ± 0.1a	6.95 ± 0.03a	7.4 ± 0.3a	6.95 ± 0.05a	6.84 ± 0.04a	7.05 ± 0.02a	6.83 ± 0.03a	6.98 ± 0.03a	7.15 ± 0.02b	5.29 ± 0.02b	7.25 ± 0.02a	6.85 ± 0.03a	7.13 ± 0.01a	6.81 ± 0.01a	0.000	0.000	0.000
Pro	3.97a	3.86 ± 0.06a	3.9 ± 0.3ab	3.65 ± 0.05ab	3.78 ± 0.04ab	3.94 ± 0.02ab	4.14 ± 0.04a	4.03 ± 0.02a	2.66 ± 0.01c	2.93 ± 0.03c	3.32 ± 0.02b	3.85 ± 0.05b	2.82 ± 0.04c	2.97 ± 0.02c	0.000	0.082	0.011
TAA	74.19 ± 1.65	75.36 ± 0.85	76.48 ± 2.16	75.38 ± 1.55	72.93 ± 0.8	75.15 ± 0.55	75.26 ± 0.85	75.82 ± 0.84	77.55 ± 0.45	71.27 ± 0.56	76.85 ± 0.56	75.61 ± 0.47	75.49 ± 0.63	73.89 ± 0.66	0.329	0.124	0.023
EAA	25.44 ± 0.33b	26.45 ± 0.29b	26.38 ± 0.68a	26.14 ± 0.45a	24.55 ± 0.17b	25.38 ± 0.25b	25.87 ± 0.35b	25.95 ± 0.32b	28.12 ± 0.17a	25.89 ± 0.17a	26.7 ± 0.28a	26.47 ± 0.2a	26.28 ± 0.26a	26.04 ± 0.34a	0.001	0.424	0.006
NEAA	48.75 ± 1.32	48.91 ± 0.56	50.1 ± 1.48	49.24 ± 1.1	48.38 ± 0.63	49.77 ± 0.3	49.39 ± 0.5	49.87 ± 0.52	49.44 ± 0.28	45.38 ± 0.39	50.15 ± 0.29	49.14 ± 0.27	49.22 ± 0.37	47.85 ± 0.32	0.062	0.068	0.043
DAA	29.06 ± 0.74	29.26 ± 0.31	30.04 ± 0.65	29.62 ± 0.86	29.53 ± 0.35	30.17 ± 0.13	29.65 ± 0.29	30.03 ± 0.28	30.72 ± 0.15	28.28 ± 0.26	30.83 ± 0.13	29.58 ± 0.12	30.74 ± 0.16	29.64 ± 0.1	0.196	0.019	0.02
EAA/TAA	0.34	0.35	0.34	0.35	0.34	0.34	0.34	0.34	0.36	0.36	0.35	0.35	0.35	0.35	–	–	–

Notes:

Different letters represent significant differences among sites for p < 0.05.

Asp, Aspartic acid; Thr, Threonine; Ser, Serine; Glu, Glutamic acid; Gly, Glycine; Ala, Alanine; Cys, Cystine; Val, Valine; Met, Methionine; Ile, Isoleucine; Leu, Leucine; Tyr, Tyrosine; Phe, Phenylalanine; Lys, Lysine; His, Histidine; Arg, Arginine; Pro, Proline. TAA, total amino acids; EAA, essential amino acids; NEAA, non-essential amino acids; DAA, delicious amino acids; EAA/TAA, essential amino acids/total amino acids.