Table 5. Evaluation of the Influenza SARS-CoV-2 Multiplex Assay specificity*.
| Virus strain | Lineage | GISAID accession no. | Con† | Cycle threshold value |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Influenza A |
Influenza B |
SARS-CoV-2 |
|||||||||||||
| Influenza A | |||||||||||||||
| A/Florida/81/2018 | A(H1N1) pdm09 | EPI1310819 | 108.1 | 13.97 | 14.00 | 14.02 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 103.1 | 27.48 | 28.16 | 27.74 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/Kansas/14/2017 | A(H3N2) | EPI1653963 | 108.5 | 13.62 | 13.66 | 13.68 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 105.5 | 25.07 | 25.00 | 25.01 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/Ohio/35/2017 | A(H1N2)v | EPI1056728 | 106.9 | 14.71 | 14.90 | 14.84 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 101.9 | 30.91 | 31.25 | 30.99 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/chicken/Pennsyl- vania/298101- 4/2004 | A(H2N2) | EPI229365 | 109.5 | 15.60 | 15.66 | 15.74 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 103.5 | 33.40 | 33.20 | 34.71 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/Ohio/13/2017 | A(H3N2)v | EPI1056648 | 106.6 | 20.85 | 20.96 | 20.86 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 101.6 | 35.48 | 35.49 | 33.98 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/canine/Florida/ 43/2004 | A(H3N2) | 108.1 | 19.61 | 19.69 | 19.44 | 0 | 0 | 0 | 0 | 0 | 0 | ||||
| EPI98471 | 104.1 | 34.10 | 35.45 | 36.87 | 0 | 0 | 0 | 0 | 0 | 0 | |||||
| A/equine/Ohio/01/ 2003 | A(H3N8) | DQ124188§ | 108.4 | 16.50 | 16.70 | 16.68 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 103.4 | 31.01 | 31.55 | 31.07 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/Northern pintail/Washington /40964/2014 | A(H5N2) | EPI860995 | 109.4 | 16.39 | 16.43 | 16.49 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 104.4 | 36.89 | 36.45 | 34.43 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/gyrfalcon/Wash- ington/41088- 6/2014 | A(H5N8) | EPI569393 | 109.75 | 14.12 | 14.10 | 14.13 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 104.75 | 29.60 | 29.38 | 29.90 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/chicken/Califor- nia/32213–1/2000 |
A(H6N2) | EPI1915583 | 109.2 | 14.97 | 15.05 | 15.08 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 103.2 | 34.19 | 32.52 | 32.49 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/feline/New York/16-040082- 1/2016 | A(H7N2) | EPI985440 | 1010.2 | 15.76 | 15.94 | 16.00 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 105.2 | 28.16 | 28.30 | 28.46 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/Taiwan/1/2017 | A(H7N9) | EPI917065 | 109.5 | 16.69 | 16.90 | 16.99 | 0 | 0 | 0 | 0 | 0 | 0 | |||
| 103.5 | 32.20 | 33.11 | 32.53 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
| A/Bangladesh/
0994/2011 |
A(H9N2) |
EPI445991 | 1010.5 | 18.03 | 18.14 | 18.21 | 0 | 0 | 0 | 0 | 0 | 0 | |||
|
|
104.5 |
34.25 |
35.32 |
36.90 |
|
0 |
0 |
0 |
|
0 |
0 |
0 |
|||
| Influenza B | |||||||||||||||
| B/Maryland/15/ 2016 | B(Victoria) | EPI1255266 | 108.5 | 0 | 0 | 0 | 13.46 | 13.49 | 13.47 | 0 | 0 | 0 | |||
| 102.5 | 0 | 0 | 0 | 30.82 | 31.07 | 31.20 | 0 | 0 | 0 | ||||||
| B/Phuket/3073/
2013 |
B (Yamagata) |
EPI1799818 | 108.9 | 0 | 0 | 0 | 13.66 | 13.67 | 13.68 | 0 | 0 | 0 | |||
|
|
102.9 |
0 |
0 |
0 |
|
31.87 |
31.77 |
32.18 |
|
0 |
0 |
0 |
|||
| SARS-CoV-2 | |||||||||||||||
| 2019-nCoV/USA- WA1/2020 | Beta-coronavirus | MT 576563 | 104.5 | 0 | 0 | 0 | 0 | 0 | 0 | 18.34 | 18.55 | 18.41 | |||
| 100.5 | 0 | 0 | 0 | 0 | 0 | 0 | 33.11 | 34.15 | 34.88 | ||||||
*Multiplex real-time reverse transcription PCR for influenza A virus, influenza B virus, and SARS-CoV-2. Evaluation conducted in triplicate. Con, concentration; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2. ‡Concentration in relation to 50% tissue culture infectious dose. §GenBank accession number.