Figure 4.

PD-1^high ILC2s expressed higher levels of IL-4 and IL-13. PD-1^high ILC2s and PD-1^low ILC2s were marked as CD45⁺Lin^-CD127⁺CRTH2⁺PD-1^high and CD45⁺Lin^-CD127⁺CRTH2⁺ PD-1^low, respectively, and sorted by FACS. (A, B) The gene expressions of PDCD1 (encoding PD-1) (A), IL4, and IL13 (B) were upregulated in the PD-1^high group compared with the matched PD-1^low group (n=26). (C–F) The representative results and statistical results of IL-4 (C, D) and IL-13 (E, F) protein expression according to the different PD-1 levels assessed by flow cytometry (for each group, n=26). Each dot represents one sample. ***P<0.001, ****P<0.0001. P values were calculated using a paired two-tailed student’s t-test.