Figure 3.

IBI323 generates clustering of cells and enhances primary T-cell activation. (a) IBI323, IBI110, Bi127, or IgG was added to a 1:1 mix of Violet-labeled PD-L1-expressing CHO-S cells and Far Red-labeled LAG-3-expressing 293-F cells. The formation of cell complexes was determined by flow cytometry. Mean percentages of double positive events are shown for each treatment group. Data are representative of three independent experiments. (b) Representative flow plot showing the percentage of double positive cell complexes. (c) Dual blockade of PD-L1/LAG-3 pathway with IBI323 enhances CD4 + T-cell function in an allogeneic MLR. IFN-γ and IL-2 secretion in culture was measured after co-culturing CD4 + T cells with monocyte-derived DCs for 3 days with IBI323, IBI110, Bi127, or IgG1. Data are derived from human PBMC from 2 healthy donors