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Schematic illustration of the proposed competitive bioassay for the simultaneous detection of A protein and NA2 target, and B two NA targets together (NA1 and NA2). The detection of a NA1 target by Aptazyme1.15-3′ was simply achieved by using the complementary sequence of TBA as the target. DNAzyme1 is always part of Aptazyme1.15-3′ and correspondingly cleaves Substrate1, whereas DNAzyme2 is part of Aptazyme2.20-5′, thereby cleaving Substrate2. TBA stands for thrombin binding aptamer
