Fig. 3.

Validation of the functionality of TBA₁₅ (A) and DNAzyme₁ (B) within the hybrid Aptazyme_1.15-3’. A FO-SPR probes were functionalized with either TBA₁₅ (positive control) or Aptazyme_1.15-3’ and their interaction with thrombin at different concentrations was monitored by a shift in the resonance wavelength. B The performance of DNAzyme₁ within the hybrid Aptazyme_{1.15- 3’} and Aptazyme_1.PolyT, the latter having TBA₁₅ replaced with a PolyT of the same length, was validated by measuring the fluorescence intensity of the cleaved F/Q — labelled Substrate₁ and compared to the original DNAzyme₁ activity. Aptazyme_1.15-3’ or DNAzyme₁ and Substrate₁ were used in these experiments at 250 nM concentration each. The error bars in A and B represent the standard deviation of two and three repetitions, respectively