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Screening of different Aptazyme2 designs based on their catalytic activity in the presence of Substrate2. The influence of different lengths (28, 20, and 15 nts) and positions (3’ or 5’ end of the DNAzyme2) of the target recognition sequences were studied. The error bars represent the standard deviation of three repetitions. Additionally, the fluorescence normalization process is described in the “Materials and methods” section
