Fig. 7.

DNA-only multiplex bioassay for simultaneous detection of different targets. Evaluation of the bioreceptor specificity in multiplex conditions using A 248 nM of thrombin and NA₂ as targets, and B 248 nM of NA₁ and NA₂ as targets. C Concentration-dependent response for the detection of thrombin and NA₂. Inset figure represents the linear range of thrombin between 0 and 124 nM (R² = 0.99) and of NA₂ between 0 and 62 nM (R² = 0.99). D Concentration-dependent response for the detection of NA₁ and NA₂. Inset figure represents the linear range between 0 and 124 nM for NA₁ (R² = 0.99) and 0 and 62 nM for NA₂ (R² = 0.99). For all graphs, the error bars represent the standard deviation of three repetitions and the LOD values have been calculated as described in the “Materials and methods” section