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. 2021 Jun 14;12:683516. doi: 10.3389/fpls.2021.683516

FIGURE 1.

FIGURE 1

Diel and circadian oscillation of mitochondrial-related gene expression. Peak phases of expression of mitochondrial-related genes represented in radial plots under (A) LDHC and (B) LL_LDHC conditions. Radial axis: 24-dawn, or subjective dawn; 12-dusk, or subjective dusk. Time course analyses by RT-QPCR of (C) ACONITASE 1 (ACO1) and (D) MITOCHONDRIAL PYRUVATE DEHYDROGENASE SUBUNIT 2-1 (PDH3) gene expression under diurnal conditions. Data are represented as the mean + SEM. Promoter activities of (E) ACO1 fused to LUCIFERASE (ACO1:LUC) and (F) ALTERNATIVE NAD(P)H DEHYDROGENASE 1 (NDA1) fused to LUC (NDA1:LUC) under diurnal and free-running conditions. Data are represented as the mean + SEM. The white and gray areas in panels (A,C–F) represent day and night, respectively. The white and dotted areas in panels (B,E,F) represent subjective day and subjective night, respectively. Three biological replicates were performed for all experiments, with plants grown independently, and with samples collected, processed and analyzed at different times.