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. 2021 Jun 14;12:683516. doi: 10.3389/fpls.2021.683516

FIGURE 2.

FIGURE 2

Miss-expression of mitochondrial-related genes in TOC1-ox and toc1-2 mutant plants. Time course analyses of mitochondrial-related gene expression in WT, TOC1-ox and toc1-2 plants over a circadian cycle. Plants were entrained under LD cycles and transfer for 2 days under LL conditions. Expression of (A) NDA1, (B) ACO1, (C) CITRATE SYNTHASE 4 (CYS4), (D) NAD-DEPENDENT MALIC ENZYME 2 (ME2), (E) NADH:UBIQUINONE OXIDOREDUCTASE FE-S PROTEIN4 (NDUFS4), (F) ACONITASE 2 (ACO2), (G) 2-OXOGLUTARATE DEHYDROGENASE E1-2 (2OXDHb), (H) DICARBOXYLATE CARRIER 1 (UCP5/DIC1) and (I) CYTOCHROME B (COB). Relative expression was obtained by RT-Q-PCR analyses. Data are represented as the mean + SEM. The white and dotted areas in represent subjective day and subjective night, respectively. Three biological replicates were performed for all experiments, with plants grown independently, and with samples collected, processed and analyzed at different times.