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. 2021 Jun 14;12:683516. doi: 10.3389/fpls.2021.683516

FIGURE 3.

FIGURE 3

TIMING OF CAB EXPRESSION1 regulates the diurnal and circadian accumulation of metabolites. (A) Heat map of GC-MS-analyzed metabolites in WT, TOC1-ox and toc1-2 plants. Plants were entrained under 12 h light: 12 h dark cycles and then transferred for 2 days to LL before sampling at CT7 and CT19. (B) Dispersion plots of metabolite fold change (day versus night) under LD (12 h light: 12 h dark cycles) and LL (subjective day versus subjective night) in TOC1-ox relative to WT. (C) Dispersion plots of metabolite fold change (toc1-2 versus TOC1-ox) under LL. Metabolites were clustered per class into organic acids (orange), amino acids (purple), and sugars and sugar alcohols (green). Relative metabolite content was normalized to the mean of WT plants and fold-change values were log2 transformed. Log2 fold differences < [0.4] were not considered (pale gray square). Orange dotted oval denotes metabolites down-regulated in TOC1-ox and up-regulated in toc1-2. Data are the result of 3–5 biological replicates, with plants grown independently at different times.