Figure 2.

Mitochondrial open reading frame of the 12S ribosomal RNA type-c (MOTS-c) prevents palmitic acid-induced atrophy in differentiated C2C12 myotubes. Differentiated C2C12 myotubes were treated with 0.5 mM palmitic acid for 48 h in the absence or presence of 50 µM MOTS-c. A: representative images of myosin (green) and Hoechst 33258 (blue; nucleus) immunostaining in C2C12 myotubes. Scale bar, 100 µm. B: Myotube number per field was quantified. Myotube diameter (n = 240 myotubes) is depicted as mean diameter (C) and a histogram of diameter distribution (D). Significant differences were determined by one-way ANOVA followed by Tukey’s post hoc test. ***P < 0.001. PA, palmitic acid.