Figure 4.

Mitochondrial open reading frame of the 12S ribosomal RNA type-c (MOTS-c) increases forkhead box protein O1 (FOXO1) phosphorylation in skeletal muscles taken from C57BL/6J mice fed a high-fat diet. A: total and phospho-FOXO1 levels (representative Western blots); GAPDH was used as a loading control. B–D: quantification of total and phospho-FOXO1 in Western blots. Data are reported as means ± SE of 12 mice per group. Significant differences were determined by Student’s t tests. *P < 0.05 and ***P < 0.001.