Figure 4.

Chitinase 3-like-1/BRP-39 promotes type 2, but not type 1 or type 17, responses during experimental fungal asthma. Balb/c wild-type (WT) and BRP-39-deficient (Chi3l1^−/−) mice were chronically exposed to A. fumigatus as described in the materials and methods. Twenty-four hours after last challenge, a bronchoalveolar lavage was performed and BAL fluid was clarified by centrifugation. A: IL-4, IL-5, and IL-13 levels were quantified in clarified BALF by Milliplex. B: IL-33, CCL17, and CCL22 levels were quantified in clarified lung homogenates by ELISA. C: IFN-γ, CXCL9, and CXCL10 were quantified in clarified BALF by Milliplex. D: IL-17A and IL-22 levels were quantified in clarified BALF by Milliplex and ELISA, respectively. E: IL-10 and TGF-β were quantified in clarified BALF by Milliplex and ELISA, respectively. F: IL-38 levels were quantified in clarified lung homogenates by ELISA. The figures illustrate cumulative data from two independent studies (n = 5 mice per group, per study). Each dot presents a single mouse. The middle line represents the mean. Data are expressed as pg/mL in lavage fluid or lung homogenate. *P value < 0.05; **P value < 0.01; ***P value < 0.001 (two-tailed Student’s t test).