Figure 6.

Presence of chitinase 3-like-1/BRP-39 in either the hematopoietic or nonhematopoietic compartment is sufficient to regulate increased AHR during fungal asthma. Bone marrow chimeras were generated in which mice expressed BRP-39 in either only the hematopoietic compartment (C57BL/6-Chi3l1^−/−; WT-KO), only the nonhematopoietic compartment (KO-WT), both compartments (WT-WT) or neither compartment (KO-KO) as described in the materials and methods. Eight to ten weeks following reconstitution, chimeras were chronically exposed to A. fumigatus as described in the materials and methods. Airway (Newtonian) resistance was analyzed via flexiVent. The figure illustrates cumulative data from two independent studies (n = 5 mice per group, per study). Data are expressed as means ± SE. ***P value  < 0.001 (two-way ANOVA). KO, knockout; WT, wild-type.