Figure 3.
Effects of neutrophil depletion and repletion on lung inflammatory injury. Endothelial cell-specific YAP knockout mice (YAP-CKO) were intraperitonially injected with isotype control immunoglobulin (Ig) G2a antibody or monoclonal anti-Ly6G antibody for 3 consecutive days. Neutrophil repletion was performed in neutropenic mice with neutrophils (2 × 106 cells/mouse, iv) isolated from wild-type (WT) or endothelial cell-specific YAP-CKO mice. All mice were ventilated with a tidal volume of 21 mL/kg for 4 h. A: neutrophil count in bronchoalveolar lavage fluid. B: protein concentrations in bronchoalveolar lavage fluid. C: levels of TNF-α in the bronchoalveolar lavage fluid. D: pulmonary vascular permeability measured by Evans blue dye extravasation. n = 6/group (three male and three female mice). **P < 0.001, two-way ANOVA with Bonferroni post hoc test. Data represent means ± SD. YAP, Yes-associated protein.