Figure 6.

Effects of YAP deletion on VE-PTP expression in lung endothelial cells. A: VE-PTP expression in lung endothelial cells analyzed by Western blot. Endothelial cells were isolated from the lungs of wild-type (WT) and endothelial cell-specific YAP knockout mice (YAP-CKO) littermates mechanically ventilated with at indicated tidal volumes (MV) for 4 h. Top: levels of VE-PTP in endothelial cells were determined by Western blot analysis. Bottom: protein quantification (normalized to GAPDH) by densitometry. B: immunohistochemical staining of VE-PTP in lung sections. Top: low-power (×20) and inset high-power (×40) magnified images of immunohistochemical staining for VE-PTP in the lungs of WT and YAP-CKO mice. Bottom: quantification of VE-PTP. n = 6 lungs (5 slices/lungs) (from 3 male and 3 female mice). All images for control and treatment groups were collected at the same time under the same conditions. *P < 0.05, **P < 0.01, two-way ANOVA with Bonferroni post hoc test. Data represent means ± SD. VE-PTP, vascular endothelial protein tyrosine phosphatase; YAP, Yes-associated protein.