Figure 8.

Exogenous expression of VE-PTP in pulmonary vasculature prevents VE-cadherin (VE-cad) phosphorylation and dissociation of VE-cadherin with catenins. Full-length VE-PTP and VE-PTP mutant (△N) cDNAs were delivered to YAP-CKO mice via cationic liposomes. Lung microvascular endothelial cells were isolated from these transfected mice. A: effects of overexpressed VE-PTP and VE-PTP mutant on tyrosine phosphorylation of VE-cadherin (VE-cad) in the endothelial cells of YAP-CKO mice. B: protein quantification (normalized to GAPDH) by densitometry. n = 6 lungs (from 3 male and 3 female mice). *P < 0.05, **P < 0.001, one-way ANOVA with Bonferroni post hoc test. C: association of VE-cadherin with p120- or β-catenin detected by immunoprecipitation after overexpression of VE-PTP and VE-PTP mutant (△N) in the YAP-CKO mice. D: protein quantification (normalized to VE-cadherin, VE-cad) by densitometry. n = 6 lung (from 3 male and 3 female mice). **P < 0.001, one-way ANOVA with Bonferroni post hoc test. Data represent means ± SD. VE-PTP, vascular endothelial protein tyrosine phosphatase; YAP, Yes-associated protein.