Table 1.
Summary of antibody validation
| Antibody Name | Company/Catalog Number | Validation | Reference |
|---|---|---|---|
| GLUT1 | Santa Cruz/sc- 377228 | Various cell lines with high/low protein expression | https://www.scbt.com/scbt/product/glut1-antibody-a-4 |
| PGAM1 | Santa Cruz/sc-130334 | Various cell lines with high/low protein expression | https://www.scbt.com/p/pgam1-antibody-6 |
| GPD1 | Santa Cruz/ sc-376219 | Various cell lines with high/low protein expression | https://www.scbt.com/p/gpd1-antibody-e-7 |
| GPD2 | Santa Cruz/sc-393620 | Various cell lines with high/low protein expression | https://www.scbt.com/p/gpd2-antibody-d-9 |
| MIOX | Santa Cruz/376080 | Positive/negative control (myo-inositol oxygenase transfected) | https://www.scbt.com/p/myo-inositol-oxygenase-antibody-e-11 |
| ZO-1 | Cell Signaling Technology/8193S | Various cell lines with high/low protein expression | https://www.cellsignal.com/products/primary-antibodies/zo-1-d7d12-rabbit-mab/8193 |
| Afadin | Cell Signaling Technology/13531S | Various cell lines with high/low protein expression | https://www.cellsignal.com/products/primary-antibodies/afadin-d1y3z-rabbit-mab/13531 |
| MKK3 | Cell Signaling Technology/8535S | Various cell lines with high/low protein expression | https://www.cellsignal.com/products/primary-antibodies/mkk3-d4c3-rabbit-mab/8535 |
| PKM2 | Cell Signaling Technology/4053 | Various cell lines with high/low protein expression | https://www.cellsignal.com/products/primary-antibodies/pkm2-d78a4-xp-rabbit-mab/4053 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | Cell Signaling Technology/9101S | Positive/negative control (MEK1, MEK1/2 Inhibitors, PDGF) | https://www.cellsignal.com/products/primary-antibodies/phospho-p44-42-mapk-erk1-2-thr202-tyr204-antibody/9101 |
| Phospho-p38 MAPK (Thr180/Tyr182) | Cell Signaling Technology/9211S | Positive/negative control (UV- or anisomycin-treated) | https://www.cellsignal.com/products/primary-antibodies/phospho-p38-mapk-thr180-tyr182-antibody/9211 |
| P38 MAPK | Cell Signaling Technology/9212S | Positive/negative control (p38 MAPK siRNA, UV- or anisomycin-treated) | https://www.cellsignal.com/products/primary-antibodies/p38-mapk-antibody/9212 |
| Phospho-AMPKα (Thr172) | Cell Signaling Technology/2535 | Positive/negative control (oligomycin- or phenformin-treated) | https://www.cellsignal.com/products/primary-antibodies/phospho-ampka-thr172-40h9-rabbit-mab/2535 |
| AMPKα | Cell Signaling Technology/5831S | Various cell lines with high/low protein expression | https://www.cellsignal.com/products/primary-antibodies/ampka-d5a2-rabbit-mab/5831 |
| Phospho-MKK3 (189) | Thermo Scientific /PA5-37700 | Positive/negative control (treated with PMA) | https://www.thermofisher.com/antibody/product/Phospho-MEK3-Ser189-Antibody-Polyclonal/PA5-37700 |
| PKM1 | Proteintech/15821-1-AP | Various cell lines with high/low protein expression | https://www.ptglab.com/products/PKM1-specific-Antibody-15821-1-AP.htm |
| Anti-PI3 Kinase p85 | Sigma Aldrich /ABS234 | Various cell lines with high/low protein expression | https://www.sigmaaldrich.com/catalog/product/mm/abs234?lang=en®ion=US |
| Anti-nitrotyrosine | Calbiochem/487923 | Various cell lines with high/low protein expression | https://www.emdmillipore.com/US/en/product/Anti-Nitrotyrosine-Mouse-mAb-CC22.8C7.3, EMD_BIO-487923?ReferrerURL=https%3A%2F%2Fwww.google.com%2F" |
| G6PD | Cell Signaling Technology/12263S | Various cell lines with high/low protein expression | https://www.cellsignal.com/products/primary-antibodies/g6pd-d5d2-rabbit-mab/12263 |
Antibodies used in the present study have undergone previous validation. GLUT1, glucose transporter protein 1; GPD1, glucose phosphate dehydrogenase 1; G6PD, glucose-6-phosphate dehydrogenase; MIOX, myo-inositol oxygenase; MKK3, mitogen-activated protein kinase kinase 3; p38 MAPK, p38 mitogen-activated protein kinase; PGAM1, phosphoglycerate mutase; PKM, pyruvate kinase isozymes; ZO-1, zona occluden-1.