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Ubiquitin carboxyl terminal hydrolase 1 (UCHL1) silencing does not affect NF-κB activation, ICAM-1, or VCAM-1 expression levels. ECs were transfected with siRNA specific for UCHL1 (siRNA, 100 nm, 3 days) or scramble siRNA (scr siRNA) prior to treatment with thrombin (1 U/mL, 15 min). Whole cell lysates were then used for Western blotting for NF-κB, phospho-NF-κB p65, ICAM-1, or VCAM-1 (representative blots shown).
