Figure 7.
Ubiquitin carboxyl terminal hydrolase 1 (UCHL1) inhibition is associated with increased VILI: BAL fluid analysis. C57Bl/6 mice were pretreated with the UCHL1 inhibitor LDN-57444 (LDN, 5 mg/kg ip) or vehicle 24 h and again 1 h before VILI challenge (VT, 30 mL/kg, 4 h). Bronchoalveolar lavage (BAL) was then collected for analysis of total protein (A) and total cell counts (B) as well as differential cell counts (C). Compared with VILI-challenged control animals, LDN-treated mice had significantly increased total protein and cell counts with significant increase specifically in PMNs (*P < 0.05, n = 3 animals/condition).