Figure 2.

Cell-free (cf)-mRNA-based high-throughput sequencing is an accurate, robust, and reproducible approach for characterizing serum transcriptome. A: histogram representing correlation between the cf-mRNA transcriptomes of technical replicates using Pearson’s correlation analysis (n = 239). B: histogram compiling r values of expected extracellular RNA consensus consortium (ERCC) transcripts vs. observed level of ERCC from each sample (n = 239). C: histogram of number of transcripts detected (transcripts per million, TPM ≥10) per sample (n = 239). D: schematics for comparison between next-generation sequencing assay and qPCR (top). Correlation between sequencing TPM against qPCR Ct value (96 genes in total, n = 12) (bottom).