Table 2.

Univariate and multivariate analysis of factors affecting DFS.

	Univariate analysis		Multivariate analysis
Variables	Odds ratio (95% CI)	P value	Odds ratio (95% CI)	P value
Sex	0.794 (0.408–1.544)	.496
Age	1.023 (0.994–1.054)	.126
Distance from anal verge (cm)	1.019 (0.974–1.067)	.405
pre-CRT CEA level (ng/mL)	1.207 (0.638–2.285)	.563
Tumour differentiation	3.351 (1.778–6.315)	<.001	1.847 (0.935–3.648)	.077
Clinical stage	1.608 (0.857–3.017)	.139
Surgery	0.997 (0.984–1.011)	.703
TRG	0.979 (0.954–1.005)	.144
ypT	6.141 (2.181–17.289)	.001	2.872 (0.951–8.673)	.061
ypN	3.365 (1.729–6.551)	<.001	2.242 (1.103–4.556)	.026
LVI	0.669 (0.295–1.517)	.336
CD45RO+ratio	0.228 (0.104–0.497)	<.001	0.339 (0.153–0.752)	.008

CEA= carcinoembryonic antigen, CRT = chemoradiotherapy, LVI = lymphovascular invasion, TRG = tumor regression grade.

The multivariate Cox analysis revealed that elevated CD45RO⁺ratio was an independent factor for better DFS (OR, 0.339; 95% CI, 0.153–0.752; P = .008).