Figure 3.

Listeria monocytogenes induce the phosphorylation of cell signaling proteins involved in TLR activation in mast cells. 2.5x10⁵ BMMC/0.25 mL of complete medium were stimulated with L.m at MOI 100:1 for different times to determine the phosphorylation of (A) ERK 1/2 at 15 minutes, (B) p38 at 60 minutes and (C) p65 at 30 minutes by flow cytometry. The graphs show the median fluorescence intensity (MFI). (Sum of 4 independent experiments, n=4 per group and for each phospho-protein. *p<0.05; Mann-Whitney test).