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. 2021 Jun 14;12:650779. doi: 10.3389/fimmu.2021.650779

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Copyright © 2021 Soria-Castro, Alfaro-Doblado, Rodríguez-López, Campillo-Navarro, Meneses-Preza, Galán-Salinas, Alvarez-Jimenez, Yam-Puc, Munguía-Fuentes, Domínguez-Flores, Estrada-Parra, Pérez-Tapia, Chávez-Blanco and Chacón-Salinas

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Cytokine production by mast cells in response to Listeriolysin-O is independent of TLR2. 2.5x10⁵ BMMC/0.25 mL of complete medium were preincubated with anti-TLR2 for 30 minutes and then were stimulated with 1000 ng/mL of Listeriolysin-O (LLO) for 24 h. Mediators levels were evaluated in culture supernatants by ELISA. (A) TNF-α, (B) IL-6, (C) IL-13 and (D) MCP-1. (Sum of 4 independent experiments, n=4 per group and for each mediator; N.D, Not detected; N.S, Not Significance; One Way ANOVA test).