Figure S6.

Cell-type-specific responses to vaccination, related to Figure 6

(A, B) Violin plot showing the upregulation of CD169 protein levels and a module of interferon response genes at day 3. Plot is similar to Figures 6C and 6D, but restricted to CD14 Monocytes, and shows the individual response of each volunteer. The response is consistent across all volunteers with one exception (P6), which exhibited signs of a highly activated immune system even prior to vaccination. (C) RNA expression of canonical interferon response gene IFI27 across the vaccination time course. The expression of IFI27 increases within particular myeloid populations at day 3, but dampens at day 7. (D) Pathway enrichment (enrichR) of the top DE genes between day 0 and day 3 myeloid cells exhibits a clear enrichment for components of the interferon response. (E) Same as in Figure 6F, but computed for cells profiled with the 10X 5′ kit.