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. 2021 Jun 24;184(13):3502–3518.e33. doi: 10.1016/j.cell.2021.04.037

Figure 6.

Figure 6

BAT Gpr3 is required for thermogenic activity in vitro but is compensated in vivo

(A) BAT thermogenic gene expression from high fat diet (HFD)-fed B-3KO mice and control littermates.

(B–E) (B) HFD-induced weight gain, (C) cold tolerance, (D) glucose tolerance, and (E) cold-induced BAT gene expression from B-3KO mice and control littermates (RT, room temperature).

(F) Norepinephrine (NE)-induced energy expenditure in anesthetized B-3KO mice and control littermates at thermoneutrality following acute cold-challenge.

(G) Indirect calorimetry of B-3KO mice and control littermates during the transition from chow to HFD.

(H–J) (H) Mitochondrial respiration, (I) fatty acid (FA) uptake, and (J) gene expression in brown adipocytes following siRNA-mediated Gpr3 knockdown.

For all panels, error bars represent ±SEM, p ≤ 0.01 = ∗∗, p ≤ 0.001 = ∗∗∗, p ≤ 0.0001 = ∗∗∗∗, t test. Box plots are presented as box: 25th to 75th percentile, and whiskers: min to max. See also Figure S5.