Figure S5.

Characterization of the C-3BO, I-3BO, AAV-modified, and B-3KO mouse models, related to Figures 5 and 6

(A) bodyweight gain of C-3BO mice and control littermates challenged with high fat diet (HFD) (independent experiment from the study in Figure 3).

(B–E) (B) food intake (average per day), (C) lean mass, (D) representative interscapular BAT images, and (E) thermogenic gene expression in scWAT of HFD-fed C-3BO mice and control littermates.

(F) thermogenic gene expression in BAT of HFD-fed I-3BO mice and control littermates.

(G and H) (G) HFD-induced energy expenditure and (H) food intake (average per day) of HFD-fed I-3BO mice and control littermates.

(I) HFD-induced day and night energy expenditure in mice infected with adeno-associated virus (AAV) particles carrying either Gfp, Adrb3, or Gpr3.

(J) schematic for conditional deletion of Gpr3 in the B-3KO mouse model.

(K) Gpr3 in situ hybridization in BAT from B-3KO and control littermates.

For all panels, error bars represent ±SEM, p ≤ 0.05 = ∗, p ≤ 0.01 = ∗∗, p ≤ 0.001 = ∗∗∗, p ≤ 0.0001 = ∗∗∗∗, t test (E, F, and I) or two-way ANOVA (A and C).