Figure S6.

Characterization of GPR3 in human thermogenic adipocytes, related to Figure 7

(A) GPR3 expression of transfected COS-7 cells for BRET-analysis.

(B–D) (B) representative light microscopy images and, (C and D), gene expression of patient-derived, non-immortalized brown adipocytes following siRNA mediated GPR3 knockdown and 4 hours vehicle or norepinephrine (NE) treatment.

(E and F)) (E) heat map of gene regulation and, specifically, (F) genes in the de novo cholesterol synthesis pathway changed by GPR3 depletion in patient-derived, non-immortalized brown adipocytes.

(G) heat map of genes induced by GPR3 activation in patient-derived, non-immortalized brown adipocytes.

(H) gene expression of patient-derived, non-immortalized brown adipocytes following siRNA mediated GPR3 knockdown and 4 hours vehicle or NE treatment.

(I) gene expression in human brown adipocytes following siRNA mediated GPR3 knockdown.

For all panels, error bars represent ±SEM, p ≤ 0.05 = ∗, p ≤ 0.01 = ∗∗, p ≤ 0.001 = ∗∗∗, p ≤ 0.0001 = ∗∗∗∗, t test.