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. 2021 Jun 18;478(12):2217–2232. doi: 10.1042/BCJ20210191

Table 1. Kinetic parameters of PGDH isozymes.

Enzyme name kcat (s−1) Kmapp (mM) kcat/Kmapp (M−1·s−1)
3-PGA NAD+ 3-PGA (×104) NAD+ (×104)
OsPGDH1 16.5 ± 0.2 0.175 ± 0.016 0.062 ± 0.007 9.4 ± 0.8 2.67 ± 0.30
OsPGDH2 30.7 ± 0.4 1.212 ± 0.053 0.348 ± 0.025 2.53 ± 0.11 0.88 ± 0.064
OsPGDH3 24.9 ± 0.3 0.519 ± 0.033 0.234 ± 0.029 4.80 ± 0.31 1.06 ± 0.13
AmtriPGDH1 20.4 ± 0.4 0.815 ± 0.070 0.245 ± 0.021 2.51 ± 0.22 0.83 ± 0.07
AmtriPGDH2 0.88 ± 0.03 3.186 ± 0.327 0.313 ± 0.038 0.0276 ± 0.0029 0.028 ± 0.003
PpPGDH1 12.1 ± 1.4 1.550 ± 0.566 0.237 ± 0.038 0.78 ± 0.30 0.51 ± 0.10
PpPGDH2 57.6 ± 2.8 5.971 ± 0.603 1.677 ± 0.068 0.96 ± 0.10 0.34 ± 0.02
PpPGDH3 56.4 ± 2.8 7.533 ± 0.661 1.156 ± 0.098 0.74 ± 0.07 0.48 ± 0.04
PpPGDH4 81.9 ± 2.8 4.392 ± 0.350 0.952 ± 0.054 1.86 ± 0.16 0.86 ± 0.05

The values were calculated from Figure 2 and are presented with the standard errors (n = 4, except OsPGDH1 (n = 3)).