Table 1. Kinetic parameters of PGDH isozymes.
Enzyme name | kcat (s−1) | (mM) | kcat/ (M−1·s−1) | ||
---|---|---|---|---|---|
3-PGA | NAD+ | 3-PGA (×104) | NAD+ (×104) | ||
OsPGDH1 | 16.5 ± 0.2 | 0.175 ± 0.016 | 0.062 ± 0.007 | 9.4 ± 0.8 | 2.67 ± 0.30 |
OsPGDH2 | 30.7 ± 0.4 | 1.212 ± 0.053 | 0.348 ± 0.025 | 2.53 ± 0.11 | 0.88 ± 0.064 |
OsPGDH3 | 24.9 ± 0.3 | 0.519 ± 0.033 | 0.234 ± 0.029 | 4.80 ± 0.31 | 1.06 ± 0.13 |
AmtriPGDH1 | 20.4 ± 0.4 | 0.815 ± 0.070 | 0.245 ± 0.021 | 2.51 ± 0.22 | 0.83 ± 0.07 |
AmtriPGDH2 | 0.88 ± 0.03 | 3.186 ± 0.327 | 0.313 ± 0.038 | 0.0276 ± 0.0029 | 0.028 ± 0.003 |
PpPGDH1 | 12.1 ± 1.4 | 1.550 ± 0.566 | 0.237 ± 0.038 | 0.78 ± 0.30 | 0.51 ± 0.10 |
PpPGDH2 | 57.6 ± 2.8 | 5.971 ± 0.603 | 1.677 ± 0.068 | 0.96 ± 0.10 | 0.34 ± 0.02 |
PpPGDH3 | 56.4 ± 2.8 | 7.533 ± 0.661 | 1.156 ± 0.098 | 0.74 ± 0.07 | 0.48 ± 0.04 |
PpPGDH4 | 81.9 ± 2.8 | 4.392 ± 0.350 | 0.952 ± 0.054 | 1.86 ± 0.16 | 0.86 ± 0.05 |
The values were calculated from Figure 2 and are presented with the standard errors (n = 4, except OsPGDH1 (n = 3)).