Table 3. Kinetic parameters of alanine-substituted AtPGDH1 enzymes.

Enzyme name	kcat (s−1)	$K_m^{\mathrm{app}}$ (mM)		kcat/$K_m^{\mathrm{app}}$ (M−1·s−1)
		3-PGA	NAD+	3-PGA (×104)	NAD+ (×105)
AtPGDH1-Q536A	42.9 ± 1.6	7.87 ± 0.55	1.22 ± 0.05	0.54 ± 0.04	0.34 ± 0.02
AtPGDH1-D538A	25.1 ± 0.4	0.51 ± 0.03	0.51 ± 0.03	4.88 ± 0.38	0.48 ± 0.02
AtPGDH1-N556A	24.4 ± 0.2	0.42 ± 0.01	0.57 ± 0.03	5.78 ± 0.26	0.42 ± 0.02
AtPGDH11	15	0.50 ± 0.05	0.050 ± 0.002	3.0	3.0

The values were calculated from Figure 5 and are presented with the standard errors (n = 4).

¹The data are cited from Okamura and Hirai [14].