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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: J Comp Neurol. 2020 Sep 11;529(7):1293–1307. doi: 10.1002/cne.25021

Figure 4: Rostral efferent neurons (RENs) are specified by hoxb1a in r4, but do not need pk1b to migrate.

Figure 4:

(a-a’) Wild-type Tg(en.crest1-hsp70l:mKaede) embryos show characteristic projections of RENs (yellow asterisk) and CENs (arrowheads) in dorsal and lateral views (n=15/15 embryos). (a”) Schematic indicates wild-type cell soma locations. (b-b”) Morpholino knockdown of hoxb1a in the Tg(en.crest1-hsp70l:mKaede) transgenic background results in a complete block to FBMN migration (yellow arrowheads); notably, RENs are absent from r6 but CENs still migrate successfully (arrowheads) (n=32/32 embryos). Otic vesicles are indicated with a dotted line. (c) In pk1b homozygous mutants, RENs (open arrowheads) and CENs (closed arrowheads) are still able to migrate, indicating that the absence of r6-localized RENs in hoxb1a depleted fish is not due to downstream effects on pk1b expression (n=5/5 embryos). Transgenic background is Tg(isl1:GFP). (c’) Morpholino knockdown of pk1b in Tg(en.crest1-hsp70l:mKaede) fish demonstrates that projections of RENs (asterisk) and CENs (closed arrowhead) are undisturbed in the absence of Pk1b (n=5/5 embryos). Otic vesicles are indicated with a dotted line. (c”) Schematic indicates soma and projection locations of all three cell types in Pk1b-depleted embryos.