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. 2021 Jun 20;2021:5546711. doi: 10.1155/2021/5546711

Figure 1.

Figure 1

The expression of UNC5B is induced in senescent endothelial cells. Senescence-associated markers were used to characterize senescent endothelial cells. (a) Representative images of SA-β-gal staining assay in young (Y) and replicative senescent (RS) HUVECs. The positive cell quantification of the two groups is shown. (b) Fold changes in expression levels of the SASP mRNAs (IL-1α, IL-1β, IL-8, MCP-1, and ICAM-1) in senescent cells compared to young cells. (c) P21, P53, and UNC5B mRNA expression in young and senescent cells. (d) The protein expression of P21, P53, and UNC5B in young and senescent cells determined by western blot analysis. Scale bar, 50 μm. Quantitative data are presented as mean ± SEM of three or more independent experiments. ∗ means P < 0.05; ∗∗ means P < 0.01.