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. 2021 Jun 20;2021:5546711. doi: 10.1155/2021/5546711

Figure 2.

Figure 2

Downregulation of UNC5B ameliorates the senescence-associated phenotype. Replicative senescent HUVECs were transfected with negative control (NC) or siRNA UNC5B. (a) Silence efficacy confirmed by detecting the mRNA level of UNC5B. (b) Representative images of SA-β-gal staining assay and the percentage rate of SA-β-gal positive cells. (c) SASP evaluated by analyzing the mRNA expression levels of IL-1α, IL-1β, IL-8, MCP-1, and ICAM-1. (d) Growth curve analysis in transfected cells at the indicated time points. (e) Number of proliferative cells as detected by the Edu incorporation assay. Scale bar, 50 μm. Quantitative data are presented as mean ± SEM of three or more independent experiments. ∗ means P < 0.05; ∗∗ means P < 0.01.