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. 2021 Jun 20;2021:5546711. doi: 10.1155/2021/5546711

Figure 3.

Figure 3

Overexpression of UNC5B promotes young endothelial cells undergoing premature senescence. Young HUVECs were infected with control lentiviral vectors (Ctrl) or UNC5B-overexpressing lentiviral vectors (UNC5B OE). (a) UNC5B mRNA levels confirmed in control cells and UNC5B-overexpressing cells. (b) The transfected cells seeded in 24-well plates for SA-β-gal staining and calculation of the percentage of positive cells. (c) Evaluation of SASP by analyzing the mRNA expression levels of IL-1α, IL-1β, IL-8, MCP-1, and ICAM-1. (d) Growth curve analysis in transfected cells at the indicated time points. (e) Number of proliferative cells as detected by the Edu incorporation assay. Scale bar, 50 μm. Quantitative data are presented as mean ± SEM of three or more independent experiments. ∗ means P < 0.05; ∗∗ means P < 0.01.