Figure 6.

UNC5B impairs endothelial function. (a) In young HUVECs as prepared in Figure 3, the tube formation was observed in 96-well plates coated with Matrigel. (b) After replicative senescent cells transfection with siRNA for three days, the cells were cultured in Matrigel medium. Representative images of morphological changes of networks are shown. Lengths of the tubes and numbers of junctions were quantitated by the Image J software. (c) Angiogenic capacity evaluated by analyzing the mRNA expression levels of VEGFA and KDR. (d) Representative images of migration assay and its quantitative analysis. Scale bar, 50 μm. Quantitative data are presented as mean ± SEM of three or more independent experiments. ^∗ means P < 0.05, ^∗∗ means P < 0.01.