Figure 5.

ChAd-SARS-CoV-2 immunization ameliorates inflammatory gene expression following SARS-CoV-2 challenge

Inflammatory gene-expression (n = 22) was quantified by RT-PCR in RNA extracted from lung homogenates 3 dpi (primer and probe sequences are in Table S1).

(A) Fold increase in gene expression for ChAd-control-immunized (IN in red and IM in black) and SARS-CoV-2-challenged hamsters.

(B) ΔΔCt values for Ifit3, Irf7, Ccl5, Cxcl10, Ddx58, and Ccl3 in ChAd-control- (open symbols) and ChAd-SARS-CoV-2-S (closed symbols)-immunized and SARS-CoV-2-challenged animals 3 dpi. ns, ^∗∗∗∗p < 0.0001, ^∗∗∗p < 0.001, and ^∗∗p < 0.01 by one-way ANOVA with a Šidák correction for multiple comparisons. Each dot is an individual animal from two experiments. Bars indicate average values.

See also Figure S7.