Table 1.

Characterization of staphylococcal isolates associated with conjunctivitis: ica gene detection profiles, phenotypic biofilm production, and antibiotic resistance results

No	Isolate no	Antibiotic resistance profile	Mean absorbance value (OD570)/ biofilm production capability**	ica genes detected by PCR
Staphylococcus epidermidis isolates
1	2 SE	Fully susceptible	0.135/weak	–
2	7 SE	TE	0.143/weak	–
3	12 SE	Fully susceptible	0.149/weak	–
4	13 SE	SXT, OFX	0.150/weak	icaA
5	17 SE	MLSB	0.142/weak	–
6	18 SE	Fully susceptible	0.144/weak
7	19 SE	Fully susceptible	0.148/weak
8	20 SE	MLSB	0.173/weak	–
9	21 SE*	FOX, TE, C	0.599/strong	icaAD,
ica operon
10	23 SE*	FOX, MLSB, TE, NOR, CIP, OFX, MXF, SXT	0.187/weak	icaAD
11	28 SE*	FOX, MLSB, NOR, CIP, OFX, LVX, MFX, GM, AN, N	0.175/weak	icaAD
12	31 SE*	FOX, MLSB, NOR, CIP, OFX, LVX, MFX, GM, C	0.161/weak	icaAD,
ica operon
13	31 I SE	FOX, MLSB	0.142/weak	–
14	31 III SE	MLSB	0.645/strong	icaAD,
ica operon
15	31VII SE	MLSB	0.252/strong	–
16	31VIII SE	FOX	0.166/weak	–
17	A SE	MLSB	0.149/weak	–
18	33 SE	Fully susceptible	0.135/weak	–
19	34 SE*	MLSB, TE, CIP, OFX, LFX, GM, AN, N, SXT	0.339/strong	–
20	35 SE*	MLSB, TE, OFX, SXT	0.552/strong	icaAD,
ica operon
21	36 SE*	FOX, MLSB, SXT	0.136/weak	–
22	39 SE	TE	0.151/weak	–
23	42 SE*	FOX, MLSB, TE, NOR, CIP, OFX, LVX, MFX, GM, N, SXT	0.184/weak	–
24	45 SE	FOX, N	0.193/weak	–
25	46 SE	MLSB	0.129/weak	–
26	49 SE	MLSB	0.128/weak	–
Staphylococcus aureus isolates
1	1SA	Fully susceptible	0.169/weak	ica D
2	3 SA	Fully susceptible	0.179/weak	ica D
3	4 SA	TE	0.162/weak	–
4	5 SA	Fully susceptible	0.348/strong	–
5	6 SA	Fully susceptible	0.157/weak	–
6	8 SA	TE, GM, N	0.189/weak	icaD
7	9 SA	Fully susceptible	0.162/weak	–
8	10 SA	Fully susceptible	0.153/weak	–
9	11 SA	Fully susceptible	0.337/strong	ica D
10	15 SA	Fully susceptible	0.273/strong	–
11	16 SA	Fully susceptible	0.206/weak	–
12	22 SA	Fully susceptible	0.200/weak	ica D
13	24 SA	Fully susceptible	0.205/weak	ica D
14	25 SA*	FOX, MLSB, NOR, CIP, C	0.212/weak	IcaD
15	30 SA*	FOX, MLSB, C	0.185/weak	IcaD
16	32 SA	Fully susceptible	0.264/strong	–
17	37 SA	Fully susceptible	0.204/weak	–
18	40 SA	Fully susceptible	0.158/weak	ica AD
19	47 SA	TE	0.468/strong	–
20	48 SA	MLSB	0.138/weak	–
21	50 SA*	FOX, MLSB, GM, AN, N	0.191/weak	icaD
22	53 SA	FOX, MLSB	0.186/weak	icaD
23	54 SA*	FOX, MLSB, NOR, CIP, OFX, LVX, MFX, AN, N	0.383/strong	–
24	56 SA*	FOX, MLSB, NOR, CIP, OFX, LVX, MFX, AN, N	0.328/strong	–
25	57 SA*	FOX, MLSB, NOR, CIP, OFX, LVX, MFX, AN, N	0.203/weak	–
26	58 SA	Fully susceptible	0.153/weak	–

^*Multidrug resistance (MDR); **OD ≤ 0.120 – non-adherent; OD > 240 – strongly adherent; OD > 120 ≤ 0.240 – weakly adherent; SA—Staphylococcus aureus, SE—Staphylococcus epidermidis, MLS_B—cross-resistance to macrolides, lincosamides, and streptogramin B, cefoxitin; detection of methicillin resistance (FOX), erythromycin (E), clindamycin (CC), tetracycline (TE), norfloxacin (NOR), ciprofloxacin (CIP), ofloxacin (OFX), levofloxacin (LVX), moxifloxacin (MXF), gentamicin (GM), amikacin (AN), neomycin (N), chloramphenicol (C), co-trimoxazole (TMP/SXT)