Fig. 3.

Computer-based evaluation considering the tumor center and the invasion zone (divided into a 50 μm wide inner zone and a 300 μm wide outer zone) with regard to their T cell content (magnification × 200). a: Two different tumors with high density of CD3 positive T-cells (black dots) separated in tumor center (1), tumor inner invasive margin (arrow 2: area between red and blue line; 50 µm) and outer invasive margin (arrow 3: area between blue and yellow line; 300 µm). b Two different tumors with low density of CD3 positive T-cells (just very few black dots) separated in tumor center (1) and tumor invasive margin (arrow 2: area between red and blue line; 50 µm) and outer invasive margin (arrow 3: area between blue and yellow line; 300 µm)