Fig. 1:

Chemical heater-based, instrumentation-free, molecular detection of SARS-Cov-2 in saliva. (A) Test workflow: (1) collect saliva sample in a tube; (2) mix saliva with lysis buffer; (3) aliquot the sample to individual tubes, each dry-storing RT-LAMP reaction mix specific to the selected target; (4) insert tube(s) in the chemical heater and add water to the EPCM to initiate exothermic reaction and heat the tubes to 60 – 65 °C for ~30 minutes. (B) Color change of the tube indicates whether the test is positive (yellow, three tubes) or negative (red, one tube). Here, the negative test is a negative control to verify that the color change does not occur because of the chemical composition of the sample.