(A) VISTA images. (B) Four-channel overlay for v-DAPI (cyan, nuclei), v-Congo red (green, for plaque core), v-GLUT1 (red, blood vessels), and v-Peripheral plaque (white) by U-net predicted high-resolution segmentation of the VISTA images in (A). (C) 6-color tandem fluorescence image of MBP (magenta, myelin basic protein) and GFAP (yellow, Glial fibrillary acidic protein) with 4-channel VISTA predictions. Scale bars: 30 μm.