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. 2021 Jun 28;11:13433. doi: 10.1038/s41598-021-92824-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TCF3 is essential for Rspo2 inhibitory effects. (A) TCF3MO rescues the anteriorized phenotype of Rspo∆T RNA overexpressing embryos. Four-cell stage embryos were dorsally injected with TCF3MO (30 ng) and/or Rspo∆T RNA (0.5 ng). Arrowheads indicate the cement gland. (B) Quantification of the data in (A) representative of two independent experiments. Numbers of embryos per group are shown above each bar. (C) Rspo∆T expression levels are not altered by TCF3MO in ectoderm explants (stage 12) in two independent experiments (Exp 1 and Exp 2). ∆T, Rspo∆T; TMO, TCF3MO.