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. 2021 Jun 28;12:4004. doi: 10.1038/s41467-021-24241-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a TRIO-tracing strategy targeting AAVretro-EF1a-Cre in PF.ul (PF upper limb), and rabies virus (AAV8-hSyn-FLEX-TVA-GFP) and Cre-dependent helper virus (EnvA G-del-Rabies-mCherry) in SC.l reveal mono-synaptic inputs to the PF-projecting neurons in the SC.l. b Raw images of injection sites in PF.ul, and SC.l with zoomed in panels of triple labels starter cells in SC (case SW190926-10A). c Rabies retrograde labeling in somatic sensorimotor cortical areas MOp and SSp-bfd. Cells labeled in the dorsal and central lateral substantia nigra reticulata (SNr), the interpolar part of the spinal nucleus of the trigeminal (SPVI) and dentate nucleus (DN) of the cerebellum. Bottom right corner is a summary diagram showing that thalamic (PF) projecting neurons in SC.l receive convergent inputs from the somatic sensorimotor cortical areas (MOp, SSp), subset of the basal ganglia (SNr), somatic sensory nuclei (i.e., SPVI), and deep cerebellar nuclei (i.e., DN).