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. 2021 Jun 28;12(7):656. doi: 10.1038/s41419-021-03938-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Heat maps of differently expressed circRNAs in PDAC tissue compared with adjacent normal tissue (GSE79634). Red indicates a higher fold-change and blue indicates a lower fold change. B circ-0005105 is produced at the SEC24A gene locus containing exon 9-12. C. RT-PCR assay with divergent or convergent primers indicated the existence of circ-0005105 in PDAC tissue. GAPDH was used as a negative control. qRT-PCR analysis of the expression of circ-0005105 after RNase R treatment (D) and Actinomycin D (E). The subcellular localization of circ-0005105 in PANC-1 and SW1990 cells were analyzed through fluorescence in situ hybridization (FISH) (F) and nuclear and cytoplasmic separation assay; Scale bar = 40 μm (G). The expression of circ-0005105 in 75 pairs of PDAC (Tumor) and adjacent normal tissues (Normal) was determined by qRT-PCR (H) and in situ hybridization (ISH); Scale bar = 400 μm (I). qPCR analysis of circ-0005105 expression in pancreatic epithelial cells (HPDE6-C7 and HPDEC) and pancreatic cancer cells (BXPC-3, CFPAC-1, MIA PACA-2, PANC-1, and SW1990). The results are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.01.