Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 28;12(7):656. doi: 10.1038/s41419-021-03938-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A The intersection of miRNAs targeted circ-0005105 predicted by circRNA bank, circAtlas, and miRNAs related to pancreatic cancer reported in the HMDD database is shown via Venn diagram. The relative expression of miR-20a-3p (B) and circ-0005105 (C) in SW1990 or PANC-1 cells was analyzed by qRT-PCR after indicated transfection. D Cells were transfected with a luciferase reporter plasmid containing wide-type (circ-0005105 Wt) or mutated circ-0005105 (circ-0005105 Mut), together with or without miR-20a-3p mimics. The relative luciferase activity was analyzed. E Immunoprecipitation of AGO2 (control, anti-IgG) in miR-20a-3p inhibitor or miR-inhibitor control transfected PANC-1 cells were performed. F The expression levels of miR-20a-3p in 75 paired pancreatic cancer tissues and non-tumor tissues were analyzed by qRT-PCR. G Pearson analysis of the correlation between miR-20a-3p expression and circ-0005105 expression in pancreatic cancer tissues. H The intersection of mRNAs potential targeting by miR-20a-3p predicted by Targetscan, miRDB, and mRNAs upregulated in pancreatic cancer reported in TCGA PAAD database is shown via Venn diagram. I Expression of candidate mRNAs were detected under treatment of miR-20a-3p mimics by qRT-PCR in PANC-1 cells. J, K Pearson analysis of the correlation between miR-20a-3p expression and candidate mRNAs expression in TCGA dataset. purple indicates p < 0.05. L, M The relative expression of COL11A1 in SW1990 or PANC-1 cells was analyzed by qRT-PCR and western blot after indicated transfection. N Cells were transfected with luciferase reporter plasmid containing wide-type (COL11A1 Wt) or mutated COL11A1 (COL11A1 Mut), together with or without miR-20a-3p mimics. The relative luciferase activity was analyzed. O The relative expression of COL11A1 in SW1990 or PANC-1 cells was analyzed by western blot after indicated transfection. P Pearson analysis of the correlation between COL11A1 expression and circ-0005105 expression in pancreatic cancer tissues. The results are presented as the mean ± SD for each group (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001.