Table 3.
Lineages-specific antibodies that label ungulate embryos.
| Lineage | Marker | Species | Antibody | Reference |
|---|---|---|---|---|
| Inner cell mass/epiblast | OCT4 | Bovine | Abcam, ab-19857 | Own unpublished observations |
| Santa Cruz, sc-8628* | (31) | |||
| Santa Cruz, sc-9081* | (25) | |||
| Porcine | Abcam, ab-27985 | (129) | ||
| Santa Cruz, sc-5279 | (26) | |||
| Santa Cruz, sc-8626* | (31, 55, 130) | |||
| NANOG | Bovine | Abcam, ab-21603 | (26) | |
| eBioscience 14-5768 | (131) | |||
| Porcine | Peprotech 500-P236 | (21, 129, 132) | ||
| Abnova, PAB6837 | (130) | |||
| SOX2 | Bovine | R&D, AF2018 | (131) | |
| Millipore, AB5603 | (30) | |||
| Biogenex, AN833-RTU | (31) | |||
| Bovine, porcine | Santa Cruz, sc-17320* | (30, 130) | ||
| Bovine, ovine, porcine | Invitrogen, 14-9811 | (7) and own unpublished observations (Figure 2) | ||
| Porcine | R&D, MAB2018 | (129) | ||
| Santa Cruz, sc-17320* | (55) | |||
| Trophectoderm | CDX2 | Bovine | Abcam, ab-74339 | (25) |
| Bovine | Abcam, ab-7848 | (31) | ||
| Porcine, bovine | Chemicon, AB4123 | (26) | ||
| Bovine, ovine, porcine | Biogenex, MU392A-UC | (7, 130) and own unpublished observations (Figure 2) | ||
| GATA3 | Bovine, ovine, porcine | Abcam, ab-199428 | Own unpublished observations (Figure 2) | |
| Hypoblast/definitive endoderm | GATA6 | Bovine | Santa Cruz, sc-9055* | (26) |
| Bovine, ovine | R&D, AF1700 | Own unpublished observations (Figure 2) | ||
| Porcine | Abcam, Ab22600 | (130) | ||
| GATA4 | Porcine | Santa Cruz, sc-25310 | (129) | |
| Santa Cruz, sc-1237* | (132) | |||
| SOX17 | Bovine, ovine, porcine | R&D, AF1924 | (7, 21, 69) and own unpublished observations (Figure 2) | |
| FOXA2 | Bovine, ovine, porcine | Cell Signaling Technology, 8186S | (69) and own unpublished observations (Figure 2) | |
| Mesoderm | T | Bovine, ovine, porcine | R&D, AF2085 | (69) and own unpublished observations (Figure 2) |
| EOMES | Porcine, ovine | R&D, MAB6166 | (Own unpublished observations) |
*
These antibodies have been discontinued.