TABLE 1.
Available laboratory assay data from each trial. Bolded data are data used in this integration.
| MAL068 | MAL071 | ||||
|---|---|---|---|---|---|
| Data type | Source lab | Reference | Data type | Source lab | Reference |
| Gene expression | |||||
| Microarray | Emory | Kazmin et al. (2017) | |||
| RNA-seq | CIDR | Du et al. (2020) | RNA-seq | CIDR, Emory | Du et al. (2020) |
| Cellular | |||||
| ICS | GSK | Ockenhouse et al. (2015); Kazmin et al. (2017) | ICS e | GSK, U Miami | Regules et al. (2016); Pallikkuth et al. (2020) |
| T-cell ELISpot | Crucell/Janssen | Ockenhouse et al. (2015) | Peripheral follicular helper cell (pTfh) frequency and function | U Miami | Pallikkuth et al. (2020) |
| Antibody | |||||
| Seroneutralization assay | Crucell/Janssen | Ockenhouse et al. (2015); Kazmin et al. (2017) | B-cell ELISpot | U Miami | Pallikkuth et al. (2020) |
| Ab-mediated opsonization | WRAIR | Chaudhury et al. (2016) | Ab-mediated opsonization | WRAIR | Chaudhury et al. (2017) |
| Ab functionality b | MGH | Suscovich et al. (2020) | Ab functionality | MGH | Unpublished |
| ELISA c | CEVAC, GSK, WRAIR | Ockenhouse et al. (2015); Chaudhury et al. (2016); Radin et al. (2016) | ELISA–Titer d | CEVAC, GSK, WRAIR, U Miami | Radin et al. (2016); Regules et al. (2016); Chaudhury et al. (2017) |
| ELISA–Avidity e | |||||
| BAMA IgG Subclass (IgG1-4) f & BLI Ab Avidity g | Duke | Seaton et al. (under revision)1 | BLI Ab Avidity h | Duke | Seaton et al. (under revision) 1 |
| BAMA and BLI IgG Subclass (IgG 1–4) i | |||||
| Luminex j | WRAIR, U Miami | Chaudhury et al. (2017); Pallikkuth et al. (2020); Pallikkuth et al. (2020) | |||
| B cell phenotyping | U Miami | ||||
| Other | |||||
| Microscopy | Ockenhouse et al. (2015) | Microscopy | Regules et al. (2016) | ||
| PCR | Ockenhouse t al. (2015) | PCR | Regules et al. (2016) | ||
Includes: T cells, peripheral T follicular helper (pTfh) cells, B cells.
Includes: antibody-dependent cellular cytoxicity (ADCC), antibody-dependent complement deposition (ADCD), antibody-dependent cellular phagocytosis (ADCP), antibody dependent dendritic cell phagocytosis (ADDCP), antibody-dependent natural killer cell activation (ADNKA), antibody-dependent neutrophil phagocytosis (ADNP), Fc array, and Ig subclass.
Includes: anti-circumsporozoite (CS), anti-Hepatitis B surface antigen (HBsAg), anti-full length CS and anti-NANP6 IgG titers; anti-CS IgG avidity.
Includes: anti-CS, anti-full length CSP, anti-C-term, anti-NANP, and anti-HBsAg IgG titers.
Includes: anti-full length CSP, anti-C-term, anti-NANP, anti-CS repeat region IgG avidity may be transferred at a later date.
Includes: binding antibody multiplex assay (BAMA) full length circumsporozoite protein (CSP), NANP6, NPNA3, C-term, and Hepatitis B subclass (IgG1-4).
Includes: biolayer interferometry (BLI) full length CSP, C-term, NANP6, NPNA3, and N-interface.
Includes: full length CSP, NANP6, NPNA3, N-interface, C-term BLI Ab Avidity data.
Includes: BAMA full length CSP IgG1-4, NANP6 IgG1-4, C-term IgG3, and Hepatitis B IgG3 and BLI full-length CSP subclass avidity.
Includes: full-length CSP, C-term, NANP6 IgG1-4 subclass titer