TABLE 2.

Bioconversion of MB9 using immobilised Escherichia coli KRX whole cells (120 mg DCW ml^–1) overproducing each enzyme separately (two-step) and overproducing both enzymes simultaneously (one-pot).

Reaction	SAHBS (mM)	Yield step 1 (%)	Compound 7 (mM)	Yield step 2 (%)	Overall yield (%)
Two-step	0.71	74	0.36	99	74
	0.76	80	0.37	97	78
One-pot	–	–	0.24	–	50
	–	–	0.44	–	91

The first step was performed in static conditions at 30°C upon addition of MB9 dye (1 cycle) or four 24-h sequential additions of MB9 dye (4 cycles). The second step was performed in stirring conditions, at 37°C, for 24 h. Reactions were performed in water.