| Antibodies |
| 4G10 (phospho-tyrosine) |
Millipore |
05-1050 |
| AKT-pS473 antibody |
Cell Signaling |
9271 |
| AKT-pT308 antibody |
Cell Signaling |
13038 |
| AKT antibody |
Cell Signaling |
9272 |
| Anti-FLAG (M2) antibody |
Sigma |
1804 |
| Anti-mouse IgG, AF-555 (Cy3) |
Invitrogen |
A31570 |
| Anti-rabbit IgG, AF-647 (Cy5) |
Invitrogen |
A31573 |
| β-actin antibody |
Cell Signaling |
4970 |
| β-catenin antibody |
BD Biosciences |
610154 |
| β-tubulin antibody |
Cell Signaling |
2146 |
| BLNK-pY96 antibody |
Cell Signaling |
3601 |
| BLNK-pT152 antibody |
Cell Signaling |
49183 |
| BLNK antibody |
Cell Signaling |
12168 |
| BTK-pT223 antibody |
Cell Signaling |
5082 |
| BTK antibody |
Cell Signaling |
8547 |
| CD19-pY531 antibody |
Cell Signaling |
3571 |
| CD19 antibody |
Cell Signaling |
90176 |
| ERK1/2-pT202/Y204 antibody |
Cell Signaling |
4370 |
| ERK1/2 antibody |
Cell Signaling |
9102 |
| FOXO1-pS256 antibody |
Cell Signaling |
9641 |
| FOXO1 antibody |
Cell Signaling |
2880 |
| H3 antibody |
Cell Signaling |
4499 |
| NFAT1 antibody |
Cell Signaling |
5861 |
| NFAT2 antibody |
Cell Signaling |
8032 |
| PLCγ2-pY1217 antibody |
Cell Signaling |
3871 |
| PLCγ2 antibody |
Cell Signaling |
3872 |
| PLCγ1-pY783 antibody |
Cell Signaling |
2821 |
| PLCγ1 antibody |
Cell Signaling |
5690 |
| Streptavidin-HRP antibody |
ThermoFisher |
21130 |
| SYK-pY352 ZAP70-pY319 antibody |
Cell Signaling |
2717 |
| SYK antibody |
Cell Signaling |
13198 |
| SYK antibody(scWB) |
Cell Signaling |
80460 |
| TBP antibody |
Cell Signaling |
8515 |
| ZAP70 antibody |
Cell Signaling |
3165 |
| Biological samples |
| Normal bone marrow mononuclear cells |
ALLCELLS |
https://www.allcells.com/tissue-products/bone-marrow/ |
| Normal peripheral blood mononuclear cells |
ALLCELLS |
https://www.allcells.com/tissue-products/leukopak/ |
| Cord blood CD34+ progenitor Cells |
ALLCELLS |
https://www.allcells.com/tissue-products/cord-blood/ |
| PDX derived B-ALL, MCL and DLBCL samples |
Weinstock Laboratory |
https://www.proxe.org/; Table S8
|
| Primary CLL and B-cell lymphoma patient samples |
City of Hope National Medical Center |
Table S8 |
| Chemicals, peptides, and recombinant proteins |
| PRT062607 (P505-15) HCl (Syk inhibitor) |
Selleckchem |
S8032 |
| Ibrutinib (Btk inhibitor) |
Selleckchem |
S2680 |
| AZD5363 (Akt inhibitor) |
Selleckchem |
S8019 |
| Doxycycline |
Clontech Laboratories |
631311 |
| Indo-1, AM |
ThermoFisher |
I1223 |
| AffiniPure F(ab’)2 Fragment Goat Anti-Human IgM |
Jackson ImmunoResearch |
109-006-129 |
| Recombinant murine IL-7 |
Peprotech |
217-17 |
| Recombinant human TPO |
Peprotech |
300-18 |
| Recombinant human SCF |
Peprotech |
300-07 |
| Recombinant human ANGPTL5 |
Miltenyi Biotec |
130-096-126 |
| Critical commercial assays |
| Duolink™ In Situ Detection Reagents Red |
Sigma |
DUO92008 |
| Small scWest Kit |
Protein Simple |
K500 |
| NE-PER Nuclear and Cytoplasmic Extraction Kit |
ThermoFisher |
78835 |
| Deposited data |
| Autoantigen arrays on Zap70Mb1-Cre mice |
This paper |
GSE148797 |
| Single-cell V(D)J sequencing from humanized mouse |
This paper |
GSE149199 |
| RNA-sequencing from Eμ-TCL1 mice |
This paper |
GSE159908 |
| Uncut immunoblots and microscopy images |
This paper; Mendeley Data |
DOI:10.17632/sdkg5xfvkx.2
|
| Experimental models: cell lines |
|
|
| KOPN8 |
DMSZ |
ACC 552 |
| SEM |
DMSZ |
ACC 546 |
| RS4;11 |
DMSZ |
ACC 508 |
| JFK51R |
Müschen laboratory |
N/A |
| REH |
DMSZ |
ACC 22 |
| ICN6 |
Müschen laboratory |
N/A |
| SUP-B15 |
DMSZ |
ACC 389 |
| Tom1 |
DMSZ |
ACC 578 |
| PDX2 |
Müschen laboratory |
N/A |
| BLQ5 |
Müschen laboratory |
N/A |
| 697 |
DMSZ |
ACC 42 |
| RCH-ACV |
DMSZ |
ACC 548 |
| Kasumi-2 |
ATCC |
ACC 526 |
| ICN-12 |
Müschen laboratory |
N/A |
| JEKO1 |
DSMZ |
ACC 533 |
| MINO |
ATCC |
ACC 687 |
| Z-138 |
DSMZ |
CRL-3001 |
| Raji |
DSMZ |
ACC 319 |
| Ramos |
DSMZ |
ACC 603 |
| Daudi |
DSMZ |
ACC 78 |
| Toledo |
ATCC |
CRL-2631 |
| OCI-LY3 |
DSMZ |
ACC 761 |
| OCI-LY7 |
DSMZ |
ACC 688 |
| SU-DHL-2 |
ATCC |
CRL-2956 |
| SU-DHL-4 |
DSMZ |
ACC 495 |
| L1236 |
DSMZ |
ACC 530 |
| L428 |
DSMZ |
ACC 197 |
| JJN3 |
DSMZ |
ACC 541 |
| LP1 |
DSMZ |
ACC 41 |
| U266 |
DSMZ |
ACC 9 |
| SFO11 |
Müschen laboratory |
N/A |
| Jurkat |
DSMZ |
ACC 281 |
| Experimental models: organisms/strains |
| Mouse: Mb1Cre
|
The Jackson Laboratory |
JAX: 020505 |
| Mouse: Rosa26LSL-Zap70
|
This paper |
N/A |
| Mouse: Syk+/Zap70
|
Konigsberger et al., 2012 |
N/A |
| Mouse: Eμ-TCL1 |
Bichi et al., 2002 |
N/A |
| Mouse: NSG-Kit-W41J/Tyr |
The Jackson Laboratory |
JAX: 026622 |
| Mouse: BCR-ABL1-tg |
>Heisterkamp et al., 1990 |
N/A |
| Oligonucleotides |
| SYK crRNA AB (for human SYK deletion) |
IDT |
CTTTTTCGCAACATCACCC |
| Zap70 crRNA AA (for mouse Zap70 deletion) |
IDT |
ACGACAGCACGTAGCCGCCC |
| CD19 crRNA AB (for human CD19 deletion) |
IDT |
AGAGCTGAAGGACGATCGCC |
| BLNK crRNA AA (for human BLNK deletion) |
IDT |
TAGTCCCTTCGAGGAACACT |
| Recombinant DNA |
| MSCV ERT2-Puro |
Müschen laboratory |
N/A |
| MSCV Cre-ERT2-Puro |
Müschen laboratory |
N/A |
| MSCV-IRES-GFP (EV) |
Müschen laboratory |
N/A |
| MSCV-IRES-puro (EV) |
Müschen laboratory |
N/A |
| MSCV-IRES-mCherry (EV) |
Müschen laboratory |
N/A |
| MSCV-BirA*-GFP |
Müschen laboratory |
N/A |
| MSCV-BCR-ABL1 |
Müschen laboratory |
N/A |
| MSCV-TCF3-PBX1-GFP |
Müschen laboratory |
N/A |
| MSCV-SYK-GFP |
Müschen laboratory |
N/A |
| MSCV-SYK-s-GFP |
Müschen laboratory |
N/A |
| MSCV-SYKY348E/Y352E-GFP |
Müschen laboratory |
N/A |
| MSCV-SYKY348F/Y352F-GFP |
Müschen laboratory |
N/A |
| MSCV-SYK-KDK402A-GFP |
Müschen laboratory |
N/A |
| MSCV-BirA*SYK-GFP |
This paper |
N/A |
| MSCV-SYK-mCherry |
This paper |
N/A |
| MSCV-ZAP70-GFP |
Müschen laboratory |
N/A |
| MSCV-ZAP70Y315E/Y319E-GFP |
Müschen laboratory |
N/A |
| MSCV-ZAP70Y315F/Y319F-GFP |
Müschen laboratory |
N/A |
| MSCV-BirA*ZAP70-GFP |
This paper |
N/A |
| MSCV-ZAP70-mCherry |
Müschen laboratory |
N/A |
| MSCV-ZAP70-puro |
Müschen laboratory |
N/A |
| MSCV-GCaMPS6-puro |
Müschen laboratory |
N/A |
| MSCV-GCaMPS6-blast |
Müschen laboratory |
N/A |
| MSCV LMP2A-GFP |
Müschen laboratory |
N/A |
| pRetroX-TET3G-Neo |
Müschen laboratory |
N/A |
| pRetroX-TRE3G-LMP2A-puro |
Müschen laboratory |
N/A |
| pTOL-hCMV-TET3G-Hygro |
Müschen laboratory |
N/A |
| pCLIP-Cas9-TRE3G-ZsGreen |
Müschen laboratory |
N/A |
| pCLIP-hCMV-tRFP-gRNA |
Müschen laboratory |
N/A |
| pCL6-GFP (EV) |
Müschen laboratory |
N/A |
| pCL6-ZAP70-GFP |
This paper |
N/A |
| Software and algorithms |
| ImageJ |
NIH |
https://imagej.net/Fiji |
| Scout |
Protein Simple |
https://www.proteinsimple.com/scout |
| QuPATH |
N/A |
https://qupath.github.io/ |
| VDJTools v1.1.7 |
Shugay et al., 2015 |
N/A |
| GraphPad Prism 7 |
GraphPad Software Inc. |
https://www.graphpad.com/scientific-software/prism/ |
| R v4.0.3 |
R Project for Statistical computing |
https://www.r-project.org |
